Aequorin蛋白
WebCells possess numerous calcium-binding proteins that regulate calcium concentration in the cytosol by buffering excess free calcium ion. Disturbances in intracellular … WebAequorin is a bioluminescent protein obtained from the jellyfish ( Aequorea victoria ). It is a 22kDa protein complex that contains bound oxygen, the luciferin coelenterazine, and …
Aequorin蛋白
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Aequorin is a calcium-activated photoprotein isolated from the hydrozoan Aequorea victoria. Its bioluminescence was studied decades before the protein was isolated from the animal by Osamu Shimomura in 1962. In the animal, the protein occurs together with the green fluorescent protein to produce … See more Work on aequorin began with E. Newton Harvey in 1921. Though Harvey was unable to demonstrate a classical luciferase-luciferin reaction, he showed that water could produce light from dried photocytes and … See more Aequorin is a holoprotein composed of two distinct units, the apoprotein that is called apoaequorin, which has an approximate molecular weight of 21 kDa, and the prosthetic group coelenterazine, the luciferin. This is to say, apoaequorin is the enzyme produced … See more Since the emitted light can be easily detected with a luminometer, aequorin has become a useful tool in molecular biology for the measurement of intracellular Ca levels. The early successful purification of aequorin led to the first experiments involving the … See more • Swiss-Prot Aequorin entry Archived 2007-03-23 at the Wayback Machine See more Aequorin is presumably encoded in the genome of Aequorea. At least four copies of the gene were recovered as cDNA from the animal. Because the genome has not been sequenced, it is unclear if the cDNA variants can account for all of the isoforms of the protein. See more Early studies of the bioluminescence of Aequorea by E. Newton Harvey had noted that the bioluminescence appears as a ring around the bell, and occurs even in the absence of air. This was remarkable because most bioluminescence reactions require See more Apoaequorin is an ingredient in "Prevagen", which is marketed by Quincy Bioscience as a memory supplement. In 2024, the US Federal Trade Commission (FTC) charged the maker with falsely advertising that the product improves memory, provides … See more WebFigure 7 水母荧光素. Figure 8 水母发光蛋白（Aequorin） 水母荧光蛋白在钙离子存在时，受氧气氧化释放出的蓝光经FÖrster循环将能量传递给GFP的发色团，随后GFP的发色团受激发释放出绿色荧光蛋白特有的绿色荧光（509 nm）。
WebAug 28, 2024 · 研究发现，绿色荧光蛋白在水母中之所以能发光，是因为水母发光蛋白aequorin在钙刺激下发生氧化反应，其产生的能量可转移到绿色荧光蛋白，刺激其发出绿色荧光。这是物理化学领域已知的荧光共振能量转移现象在生物体中的首次发现。 种类繁多的 … WebAequorin 作为检测钙离子新型探针的使用,极大地推动了人们对Ca 2+ 在细胞信号转导过程中作用的认识进程。. 水母发光蛋白由189个氨基酸组成,具有3个Ca 2+ 结合的EFhand结 …
http://www.bioon.com.cn/doc/showarticle.asp?newsid=71724 WebAequorin is a calcium-sensitive bioluminescent protein from the jellyfish Aequorea victoria that has been used extensively as a calcium indicator in cells. The aequorin complex emits blue light when bound to calcium ions. The luminescence intensity is directly proportional to the Ca2+ concentration. Our coelenterazine-based kit is much more ...
Web從那裡開始，他逐步發現發光物質為單一蛋白質，他稱之為「aequorin」（譯作水母發光蛋白或水母素）[2] — 發出藍光的蛋白質。 ... 如果GFP可以與目標蛋白連接，然後在有高 …
WebAequorinはGFP同 様オワンクラゲから分離精製された カルシウム結合性発光蛋白質で,カ ルシウムがAequorin に結合すると基質であるセレンテラジンを分解し,そ の過 程で発光が起こる。化学発光であるため励起光を必要とし ないが、基質を補ってやる必要がある。 movingart.comhttp://yph-bio.com/users/20100419161814882.pdf moving artisan 6 to a new computerWebJun 7, 2024 · Aequorin的生物光峰值位于叶绿素b最大吸收波长范围。为减少拟南芥幼苗中叶绿素的干扰，研究人员将Aequorin报告系统引入到具有白化子叶表型的sig6突变体中， … moving art netflix musicWeb水母发光蛋白复合物（Aequorin）由22kDa的水母蛋白（Apoaequorin protein）、分子氧和底物腔肠素组成。只有当钙离子（Ca2+）与该复合物结合后，腔肠素才能被氧化生成高 … moving art netflix the last of usWeb水母素（英語： Aequorin ，又譯埃奎明，分子量：相對分子約35000）是一種生物发光 蛋白质，屬荧光素酶，具蛋白質一般特性，双缩脲和茚三酮反應均呈陽性，遇硫酸铵時沉澱 … moving artsWebJan 1, 2014 · Aequorin was the first Ca (2+)-sensitive photoprotein probe used to measure the [Ca (2+)] inside specific intracellular organelles in intact cells. Aequorin is a 22-kDa … moving arts danceWebAequorin definition, a protein, secreted by certain jellyfish, that produces a blue light upon binding with calcium. See more. moving art i can post to fb