Hcrt-cre

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August undefined, 2024

WebAug 2, 2024 · Hcrt-Cre transgenic mice and C57BL/6J mice were used to purify HCRT and MCH cells, respectively. In this method, dissociated hypothalamic cells are dispensed in … WebOrexin receptors and dopaminergic neurones in the VTA were identified using immunofluorescence. Orexinergic terminals in the VTA were optogenetically regulated to detect the endogenous orexin-mediated regulation of dopaminergic neurones during anaesthesia in Hcrt cre rats.

Tg(HCRT-cre)1Stak Transgene Detail MGI Mouse …

WebNov 19, 2024 · The retro-tracing virus was restrictedly injected into the bilateral VTA in Hcrt-Cre mice (Fig. 1 e and f). Half of the virus-injected mice were then subjected to CUMS for 21 days, and the others were housed under SPF conditions for control. All CUMS and control groups were simultaneously killed for immunofluorescence staining. WebJan 1, 2024 · Hcrt-cre rats were killed in the fifth week after virus injection for confirmation of virus expression and fibre location, immediately after completion of photostimulation … email for scott perry

Glp1r-ires-Cre… Harvard Office of Technology Development

WebCre mice grouped by gene. (291 Cre lines of which 246 have images.) In collaboration with the Intramural Program of the National Institute of Mental Health (PI: Dr. Charles Gerfen), GENSAT offers transgenic BAC-Cre recombinase driver lines that allow for cell-specific gene manipulations in the central nervous system of the mouse. http://www.informatics.jax.org/recombinase/summary?driver=HCRT Web摘要：目的:研究小鼠下丘脑外侧区(LHA)内食欲素(orexin)神经元向腹侧被盖区(VTA)的投射及VTA靶神经元的类型及其所占比例.方法:利用脑立体定向技术将逆向示踪病毒AAVretro-DIO-mCherry注射至Hcrt-Cre小鼠的VTA,待病毒表达之后制备包含LHA的间脑切片,用免疫荧光染色法进行orexin阳性神经元染色,计算病毒表达 ... email for second interview invitation

The evolutionarily conserved miRNA-137 targets the neuropeptide

Hcrt-cre

Selective optogenetic activation of orexinergic terminals …

WebCre-mediated excision of the TD restores expression under the direction of the native promoter. 027707 B6.129- Hcrtr2 tm1Tesc /J A floxed transcription-disrupter (TD) ablates expression of the mouse Hcrtr2 gene in this OX2R TD conditional mutant strain. Cre-mediated excision of the TD restores expression under the direction of the native promoter. WebGlp1r signaling regulates insulin secretion and gastric and small bowel motility (Cell, 2016). GLP1 is known as a clinically potent anti-diabetic and anti-feeding hormone and Glp1r-ires-Cre mice allows for specific targeting of cells that detect GLP1. The mouse strain can be used as a tool to study: - Gut motility. - Gastrointestinal function.

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WebJul 23, 2024 · Hcrt-LH and LepRb-LH neurons: physiology and behavior. To establish selective Hcrt-LH targeting, we developed a knock-in IRES-Cre mouse line (Hcrt-Cre; Supplementary Fig. 1a,b).Following intra-LH ... Web023418 B6.Cg-Tg(HCRT-MJD)1Stak/J Hypocretin (orexin) containing neurons are ablated in these transgenic mice, which may be useful in studies of narcolepsy, regulation of …

WebJun 9, 2024 · C57BL/6J mice (4–6 months old) expressing GFP (Hcrt-GFP) (18, 38, 39, 44) or Cre-recombinase (Hcrt-Cre) selectively in hypocretin neurons under the control of a specific hypocretin promoter were used in this study. Mice were group housed and maintained on a 12-hour light/12-hour dark cycle (lights on at 6:00 am) with free access … WebSelecting multiple terms will filter for results with any (i.e. logical OR) of the selected terms.

WebThe HCR file extension indicates to your device which app can open the file. However, different programs may use the HCR file type for different types of data. While we do not … WebMutation details : Bacterial artificial chromosome clones containing the preprohypocretin (Hcrt) gene were used to target a cassette containing the internal ribosomal entry …

WebSep 2, 2016 · Carbapenem-resistant Enterobacteriaceae (CRE) are multidrug-resistant gram-negative bacilli that can cause infections associated with high case fatality rates, and are emerging as …

WebMay 1, 2024 · Using a Hcrt-Cre transgenic mouse line combined with optogenetic release of Hcrt in a temporal manner, we have shown antagonism of HcrtR2 mainly mediates the … email for sending a resumeWebImages for hypocretin ( Hcrt ) TRAP (translating ribosome affinity purification) founder line JD218 This line is available from The Jackson Laboratory, stock ID 028619 Click on a thumbnail of mouse brain to examine the image in detail. email for sending an invoice email for senator rick scottWebCRE are Epidemiologically Important for Several Reasons: • Invasive infections (e.g., bloodstream infections) caused by CRE have been associated with high mortality rates … ford pass connect 4g wifi costWebMeaning. HCRT. High Country Rail Trail (Australia) HCRT. Hyperthermo-Chemo Radio-Therapy. Note: We have 2 other definitions for HCRT in our Acronym Attic. new search. … email for select portfolio servicingWebMethods: Hcrt cre rats were generated using a CRISPR/Cas9-based approach. Viruses encoding optogenetic probes were injected into the perifornical lateral hypothalamic … email for senator scott floridaWebNov 27, 2024 · Hcrt deficient mice lacking one copy of the 5HTT gene ... DR neurons of mice harboring loxP site-flanked alleles of both HCRT receptors 1&2 genes 27 with AAV expressing mCherry and Cre recombinase. fordpass connect - embedded modem