Irf8 flox mouse

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August undefined, 2024

WebMar 8, 2024 · SSc remain unknown. IRF8 is a transcriptional regulator that is essential for the differentiation and function of monocytes and macrophages and thus may be involved in the regulation of macrophage phenotypes in SSc fibrosis. In this study, we measured IRF8 levels in circulating monocytes of 26 patients with SSc (diffuse cutaneous SSc, WebIRF8 is a transcriptional regulator that is essential for the differentiation and function of monocytes and macrophages and thus may be involved in the regulation of macrophage …

IRF8 - an overview ScienceDirect Topics

WebDuring our analyses, we also examined littermate mice with a genotype of floxed IRF8 only, PU.1 only, or IRF8/PU.1 heterozygous with or without Mb1-Cre (SI Appendix, Table S1). While Mb1-Cre–mediated PU.1 deletion alone did not affect B cell distribution in the BM, Mb1-Cre–mediated IRF8 deletion alone appeared to induce a moderate reduction ... WebJan 1, 2014 · Here we show that IRF8 is downregulated in both mouse hearts and cardiomyocytes after hypertrophic stress treatment. Moreover, IRF8-deficient mice reveal an aggravated pressure overload-induced hypertrophic response, whereas the opposite effects occur in IRF8-overexpressing mice. biotic part of soil

Tnfrsf8-Flox

WebFeb 7, 2024 · IRF8 is required for the development of lymphoid CD8α + DCs, but nothing is known about its requirement for aortic DCs. We reconstituted lethally irradiated Ldlr–/– … WebIRF8 and IRF1 are transcriptional regulators that play critical roles in the development and function of myeloid cells, including activation of macrophages by proinflammatory signals … WebThe Kynu gene ( NCBI Reference Sequence: NM_027552.2 ; Ensembl: ENSMUSG00000026866 ) is located on mouse chromosome 2. Fourteen exons are identified , with the ATG start codon in exon 2 and the TAG stop codon in exon 14 (Transcript Kynu- 201: ENSMUST00000028223). Exon 3 will be selected as target site. Cas9 and gRNA will … biotic parts

Transcription factors IRF8 and PU.1 are required for …

Frontiers IRF8-Dependent Type I Conventional Dendritic Cells …

WebMyeloid cell-specific IRF8 conditional knockout mice (IRF8flox/flox; Lyz2Cre/+) with a C57BL/6 background were generated by mating IRF8-flox mice (Feng et al. 2011) with Lyz2-Cre knock-in mice. The primers used for genotyping are shown in Supplementary Table S3. The genotyping results are shown in Supplementary Figure S4a. Harvesting of blood ... WebUpon arrival, some Irf8 f mice were bred with B6.Cg-Tg(Sox2-cre)1Amc/J mice (Stock No. 008454) for germline removal of floxed exon 2. Offspring with pan deletion of floxed exon … biotic picsWebLocomotion in Nkx6.2-null mice (n = 10) was characterized by an increased number of steps during the same period of time compared to control mice (n = 15), without any change in … biotic pollinating agents

"Web014175 B6.Cg- Irf8 tm1.1Hm /J This Irf8 f floxed targeted mutation strain may be useful in generating conditional mutants for studying B cell differentiation, immunodeficiency … " - Irf8 flox mouse

Irf8 flox mouse

WebInterferon regulatory factor 8 (Irf8) is a transcription factor that negatively regulates osteoclast differentiation and Irf8 global knockout (Irf8 -/-) mice have been shown to have …

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WebFeb 11, 2024 · Mechanistically, loss of SENP3 increases interferon regulatory factor 8 (IRF8) SUMO3 modification at the K310 amino acid site, which upregulates expression of the nuclear factor of activated T cell c1 (NFATc1) and osteoclastogenesis. WebCopies of IRF8 flox or delta locus/ reference gene locus CD45.2 (normalized) ... Mononuclear phagocyte composition in Itgax-cre.Irf8fl/fl or fl/- and Irf8fl/fl mice and effect of Irf8 haplo-insufficiency on intestinal DC subset composition, related to Figure 1. (A and C) Representative flow cytometry plots and (B and D) ...

WebIrf8 cKO mice were generated by crossing Irf8 fl/fl mice with Csf1r cre mice, a monocyte/macrophage targeting Cre driver mice. ... Monocyte Subsets with High Osteoclastogenic Potential... WebIRF8 Model Description These mice carry loxP sites flanking exon 3-6 of Irf8 gene. When crossed with a Cre recombinase-expressing strain, this strain is useful in eliminating tissue-specific conditional expression of Irf8 gene. Related Strain (s) Irf8-KO Strain Name： C57BL/6JSmoc- Irf8em1Smoc

WebMar 8, 2024 · Because IRF8 may contribute to SSc progression, including fibrosis, vascular abnormalities, and impaired wound healing, we evaluated IRF8 levels in SSc monocytes … WebThe Transgenic and Knockout Mouse Shared Resource provides members of the DCI with comprehensive and user-friendly services for the production of custom designed, …

WebInterferon regulatory factor (IRF) 8 is a transcriptional regulator that is essential for the differentiation and function of monocytes and macrophages, and thus may be involved in the regulation...

WebDec 11, 2024 · Mice with IRF8 deficiency only in colon epithelial cells (termed IRF8cKO) were created by crossing Irf8-floxed mice with villin-cre mice (Figures S1A and S1B) with the deletion of exon 2 (Figure S1C). As reported in the literature, we observed that IRF8KO mice exhibit elevated levels of CD11b + Gr1 + MDSCs as compared to wild-type (WT) mice. … biotic plastWebSecukinumab (anti-IL-17A) (Cosentyx, AIN457) is a human IgG1κ monoclonal antibody that binds to the protein interleukin (IL)-17A, and is applied for the treatment of psoriasis, ankylosing spondylitis, and psoriatic arthritis. MW=147.9 kDa. Selleck's Secukinumab (anti-IL-17A) has been cited by 2 Publications biotic organism characteristicsWebFigure Legend Snippet: Upregulation of downstream targets of ERK pathway. A. Western Blot analysis of protein extract from normal appearing mucosa obtained from small intestines tissues from LSL-K-RasG12D and Villin-Cre/LSL-K-RasG12D mice. 10 ug of total protein extracts from LSL-K-RasG12D and Villin-Cre/LSL-K-RasG12D were analyzed by Western … biotic physical weatheringWebIrf8 −/ mice exhibited multiple deficiencies in myeloid and lym-phoid systems, including excessive generation of myeloid cells and diminished Th1 immune responses (37–39) … biotic petsWebThe Irf8 floxed targeted mutated mouse strain can be used to selectively ablate expression of IRF8 in any cell type in which a Cre recombinase gene is activated. This will permit the... dakota meadows middle school north mankato mnWebOct 1, 2024 · The IRF8 FLOX mice (C57BL/6J background) were kindly provided by professor Hongliang Li (Wuhan University, China). The hepatocyte-specific IRF8-knockout mice … dakota men\u0027s wrist watchesWebJun 21, 2024 · IRI was induced in Irf8-deficient mice (Irf8 fl/fl Clec9a cre mice) and control mice (Irf8 fl/fl Clec9a wt mice). (A) mRNA expression of chemokine receptors C-C … biotic plant diseases of turf