Phospho mtor antibody

Author: fhoc

August undefined, 2024

WebFor Analyzing Cell Signaling Pathway Activation. Phosphorylation, the addition of a phosphate group to serine, threonine or tyrosine residues, is an important cellular process utilized to send cellular signals from the membrane to the nucleus. For example, changes in physiological conditions may require immediate changes in gene expression. WebWhen selecting a phospho-specific antibody for your experiments, it is crucial to ensure that the antibody specifically detects the protein of interest only when it is phosphorylated at the indicated site. It is also essential to check that the antibody detects a band at the correct molecular weight for your phosphorylated target protein.

Anti-mTOR (phospho S2448) antibody (ab1093) Abcam

WebMTOR, also named as FRAP1, FRAP, FRAP2 and RAPT1, belongs to the PI3/PI4-kinase family. MTOR is a Ser/Thr protein kinase that functions as an ATP and amino acid sensor … Web4% PFA plus methanol. Methanol only. This clone failed to immunoprecipitate EGFR Phospho (Tyr845) from whole cell extracts prepared from EGF-stimulated A-431 cells. This recombinant antibody clone is derived from clone A18028A. This clone is predicted to recognize the region corresponding to EGFR Phospho (Tyr845) in both rat (Tyr846) and … d1 sharepoint

Phospho-mTOR (Ser2448) antibody (67778-1-Ig)

WebProtocol Outline. Prepare all reagents and samples as instructed in the manual. Add 100 µl of sample or positive control to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 µl … WebThe Phospho-mTOR (Ser2481) Antibody from Cell Signaling Technology is a Rabbit Polyclonal antibody to MTOR. This antibody recognizes Human, Mouse, Rat, and Non-Human Primate antigen. The Phospho-mTOR (Ser2481) Antibody has been validated for the following applications: Western Blot. WebMTOR, also named as FRAP1, FRAP, FRAP2 and RAPT1, belongs to the PI3/PI4-kinase family. MTOR is a Ser/Thr protein kinase that functions as an ATP and amino acid sensor to balance nutrient availability and cell growth. MTOR is kinase subunit of both mTORC1 and mTORC2, which regulate cell growth and survival in response to nutrient and hormonal ... d.a. schaffrick law

Phospho-mTOR (Ser2448) (D9C2) XP ® Rabbit mAb #5536

Phospho-mTOR (Ser2448) Antibody Cell Signaling Technology

WebDec 28, 2024 · Phospho-mTOR (Ser2481) Antibody Catalog Number: 2974S Clone Number: N/A Info: Supplier Page View Product Specs Browse Similar Products: MTOR Antibodies Cells (fibroblast, MRC-5 human cell lines) were starved form 4 hours (0,5% FBS) and induced with TGF-b. eIF4E siRNA was transfected with lipofectamine RNAmax for 24 hours. Web2884) and total mTOR antibodies were diluted 1:1000. Phospho-S6K (Thr-389), Phospho-S6K (Thr-421/424), total S6K1, phospho-4E-BP1 (Thr-37/46), and total 4E-BP were diluted 1:1000 (Cell Signaling Technology, Beverly, MA). β-actin antibody was diluted 1:5000 and FKBP12 antibodies were diluted 1:200 (Santa Cruz, Santa Cruz, CA). Anti-rabbit and ... d and d insulatorsWebAnti-phospho-mTOR (Ser2159) Antibody Anti-mTOR Antibody detects level of phospho-mTOR (Ser2159) & has been published & validated for use in Western Blotting, Peptide Inhibition Assay, Dot Blot. - Find MSDS or SDS, a COA, data sheets and more information. d and m electric bowling green ky

"WebDownload Zoom Anti-mTOR Antibody is an antibody against phospho-mTOR (Thr2164) for use in Dot Blot, Western Blotting. Anti-phospho-mTOR (Thr2164) Antibody MSDS … " - Phospho mtor antibody

Phospho mtor antibody

PhosphoPlus ® mTOR (Ser2448) Antibody Duet #39182

WebSep 5, 2024 · p-mTOR Antibody (59.Ser 2448) is an IgG1 κ mouse monoclonal p-mTOR antibody that detects Ser 2448 phosphorylated mTOR of mouse, rat and human origin by … WebApr 14, 2024 · Neuropathic pain caused by trigeminal nerve injury is a typical refractory orofacial chronic pain accompanied by the development of hyperalgesia and allodynia. We previously demonstrated that the mammalian target of rapamycin (mTOR) inhibitor rapamycin suppressed orofacial formalin injection-induced nociception; however, the …

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WebSupplier Page from Biorbyt for mTOR (phospho-T2446) peptide. Product Specs; Company Info; Reviews; Product Specs; Item mTOR (phospho-T2446 ... This peptide was used as immunogen to produce orb338873 (mTOR (phospho-T2446) antibody)The quality of the peptide was evaluated by reversed-phase HPLC and by mass spectrometry. Add to … Web2884) and total mTOR antibodies were diluted 1:1000. Phospho-S6K (Thr-389), Phospho-S6K (Thr-421/424), total S6K1, phospho-4E-BP1 (Thr-37/46), and total 4E-BP were diluted …

WebRabbit Phospho-mTOR-S2448 Rabbit mAb (AP1413), validaed in WB,IHC and tested in Human,, Mouse. 100% Guaranteed. ABclonal provides trial size antibody samples for … WebDec 28, 2024 · Phospho-mTOR (Ser2481) Antibody Catalog Number: 2974S Clone Number: N/A Info: Supplier Page View Product Specs Browse Similar Products: MTOR Antibodies …

WebKey features and details. Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply. Rabbit monoclonal [EPR426 (2)] to mTOR (phospho S2448) - BSA and Azide free. Suitable for: IHC-P, … WebAnti-phospho-mTOR (Thr2446) Antibody Upstate®, from rabbit; Synonyms: FRAP1, FRAP, FRAP2, RAFT1, RAPT1, FK506 binding protein 12-rapamycin associated protein 1; find …

WebMTOR, also named as FRAP1, FRAP, FRAP2 and RAPT1, belongs to the PI3/PI4-kinase family. MTOR is a Ser/Thr protein kinase that functions as an ATP and amino acid sensor …

WebProduct name Anti-mTOR (phospho S2481) antibody [EPR427 (N)] See all mTOR primary antibodies Description Rabbit monoclonal [EPR427 (N)] to mTOR (phospho S2481) Host species Rabbit Tested applications Suitable for: WB, Dot blot more details Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP Species reactivity Reacts with: Human d and s tracksWebPhosphoPlus® mTOR (Ser2448) Antibody Duet PhosphoPlus ® mTOR (Ser2448) Antibody Duet #39182 Reviews () Citations (2) Flow cytometric analysis of A549 cells using mTOR (7C10) Rabbit mAb (solid line) compared to concentration-matched Rabbit (DA1E) mAb IgG XP ® Isotype Control #3900 (dashed line). d art demon slayerWebtracts were immunoprecipitated with rabbit -mTOR antibody or-AU1 antibody. Immune complexes were collected on protein A-Sepha-rose (Sigma) or -mouse IgG-agarose (Sigma), washed three times with ... tion—Previous reports demonstrated that mTOR was phospho-rylated in vitro by AKT at a serine residue (Ser-2448) located in studies, we ... d and h collisionWebProtocol Outline. Prepare all reagents and samples as instructed in the manual. Add 100 µl of sample or positive control to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 µl of prepared primary antibody to each well. Incubate 1 h at RT. Add 100 µl of prepared 1X HRP-Streptavidin to each well. d12 meaningWebAug 24, 2005 · Anti-mTOR (phospho S2448) antibody See all mTOR primary antibodies Description Rabbit polyclonal to mTOR (phospho S2448) Host species Rabbit Tested applications Suitable for: IHC-P, WB more details … d and m past paperWebThis affinity purified antibody has been tested for use in immunohistochemistry, ELISA and western blotting. Western blotting shows reactivity specific for phospho mTOR detecting a band at approximately 250 kDa. Reactivity in other immunoassays is unknown. Purity/Specificity d2 buck\u0027s-hornWebWestern blot analysis of NIH/3T3, using Phospho-mTOR-S2448 Rabbit mAb (AP1413) at 1:1000 dilution.NIH/3T3 cells were treated by EGF (100 ng/ml) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in … d and w fresh market petoskey mi